Free FACS software. Does anyone know free software to download for windows that can open fsc files? A Becton Dickinson FACS Aria™ cell sorter with an Aerosol Management System was run in.
Summary Here we describe all procedures depicted in more detail. Part 1: How to extract high-resolution dot plots and histograms from FACSDiva Part 2: How to generate histogram overlays (based upon Part 1) Part 3: How to delete quadrant labels (Q1–Q4) within dot plots (based upon Part 1) Part 1: A detailed description of Diva-Fit - how to extract high resolution dot plots and histograms from BD’s FACSDiva software The FACSDiva software itself is unable to export any high-resolution image files; neither copy/paste, nor Export Worksheet Elements result in satisfying image quality. Interestingly, even the new built-in Save as PDF function of FACSDiva is not able to generate such high resolution, vector-based files – instead it combines the low-resolution raster/pixel graphics in a PDF file.
However, FACSDiva supports high-resolution printing, as can easily be seen on any printout generated with the software. In this supplement a detailed description shows how FACSDiva can be “tricked” into producing high-resolution images, using its print functionality and a vector-based PDF file as intermediate step. Below we describe the Diva-Fit procedure step-by-step. With some experience, even the generation of high-resolution histogram overlays will only take a few minutes using Diva-Fit.
Our proposed strategy (Diva-Fit) requires several programs to be installed on the computer. We made sure that, with the exception of FACSDiva, the required software is free of charge and can be easily obtained via download. Administrative rights are necessary to install the software.
If you do not have enough rights to install software, ask your local system administrator to install the following software tools. • BD FACSDiva 6.1.2 (any version should work) • PDFCreator 0.9.5 This version or newer is recommended. Alternatively, other PDF printer drivers possibly already installed on the computer could be used (e.g., Adobe Acrobat comes with a PDF printer driver; the free Acrobat Reader does not).
However, the method will be different. • Adobe Acrobat Reader 9.1.0 We recommend this version or newer.
Older versions may not provide all the features required. Step 1: Obtaining and processing data with FACSDiva Prepare your FACS analysis in FACSDiva as usual. This example shows cells expressing a fusion protein consisting of EGFP and Zeo R, rendering the cells EGFP positive and resistant to zeocin (lower panels after selection).
Importantly, if a histogram overlay needs to be created, it is essential to make the histograms the same size and to set the scaling of the Y-axis to identical values. To do so, mark both histograms and click the button Make Same Size. Then go to the Inspector ( View --> Inspector) and at the Histogram tab, check Manual and Counts, then enter an appropriate value. Step 2: Generation of high-resolution PDF files using PDFCreator To convert the work sheet into a high resolution PDF file, click on File --> Print. Select PDFCreator as the printer and click OK. When the dialog of PDFCreator shows up, click Options to configure how the PDF files will be generated. This only has to be done the first time.
In the Formats menu, click on PDF, then on the Compression tab. Adjust the compression settings according to the picture below (check Compress Text Objects and check Compress for all three types of images, always select ZIP as compression mode). Make sure Resample is always unchecked. Now click Save to get back to the previous dialog. Click Save to save the PDF file and select the folder where the file should be stored. Step 3: Export of images into a desired application (e.g., presentation tool such as PowerPoint) using Acrobat Reader Open the PDF file in Adobe Acrobat Reader to select and copy the desired plots to any other application.
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This PDF file contains vector graphics and even at high levels of zoom the text will be perfectly sharp and not pixelated. The next two pictures show a comparison between the PDF file created by PDFCreator and a PDF file created by FACSDiva using its Save as PDF function – the difference is obvious. Additionally, these PDF files are well suited to archival for later use or to be sent by email as they can be easily viewed without the need to have FACSDiva installed. Below you see the PDF file containing vector graphics zoomed to 1200%.
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