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Akt uplotneniya peska blank texas. Because the pan-anti-B55 antibody preferentially reacts with the α isoform, we next investigated the possible association of PP2A AB55αC holoenzyme (hereafter referred to as PP2A-B55α holoenzyme) with Akt. HA-tagged B55α (B55αHA) was ectopically expressed in NIH3T3 cells and subsequently pulled down by anti-HA antibody. Significantly, Akt was co-immunoprecipitated with B55 subunits (), and as expected, A and C subunits were also co-immunoprecipitated with B55 subunits (). Co-immunoprecipitation analyses of B55αHA-associated complexes revealed that endogenous Akt was co-immunoprecipitated with PP2A-B55α holoenzymes in cells expressing B55αHA but not in parental NIH3T3 cells ().
Created Date: 3/6/2016 2:49:13 PM. 2019-01-18 -Waterprof-USB-Socket-Lighter-Charger-Power-Adapter-Splitter/951784930.
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